Figure 7

Effects of tamoxifen, 4-hydroxytamoxifen and deficiency of D -(+)-glucose, L -leucine, L -methionine or L -cysteine on the expression of mitochondrial ATP5A and SIRT3 in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen, 4-hydroxytamoxifen and (b and e) deficiency of L-leucine, L-methionine or L-cysteine on the expression of mitochondrial ATP5a in the Complex V of respiratory oxidation-phosphorylation system. (c and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen, 4-hydroxytamoxifen and (d and e) deficiency of L-leucine, L-methionine or L-cysteine on the expression of mitochondrial SIRT3. The SIRT3 is one of the seven mammalian anti-aging and anti-metabolic sirtuins. All assays were performed in triplicates and repeated three times.