Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

Table 2 Fusion of homologous and non-homologous cells: mean ± standard deviation.

Cell sample	Number of cells (× 10⁶)	Bi-tri nucleated cells (%)	Poly nucleated cells (%)	Single-dye polykaryocytes (%)	Dual-dyes polykaryocytes (%)
Ly-Ly 1 ^st step	98 ± 3.4	1.3 ± 0.8	0.11 ± 0.22	1.44 ± 0.24	0.35 ± 0.04
Ly-Ly 2 ^nd step	17.9 ± 0.6	79.61 ± 0.86	8.9 ± 5.2	9.5 ± 1.2	7.73 ± 2.5
Er-Er 1 ^st step	1 ± 0.06	0.76 ± 0.01	1.04 ± 0.8	0.74 ± 0.05	0.33 ± 0.66
Er-Er 2 ^nd step	7.02 ± 0.11	3.3 ± 0.5	2.9 ± 0.95	2.8 ± 1.09	1.44 ± 0.87
Ly-Mo 1 ^st step	99 ± 0.91	0.48 ± 0.05	0.24 ± 0.01	0.25 ± 0.09	0.22 ± 0.03
Ly-Mo 2 ^nd step	70.07 ± 1.85	2.23 ± 0.02	1.75 ± 0.84	1.63 ± 0.78	0.71 ± 0.06

1000 cells were counted in phase contrast microscopy and in fluorescent microscopy using a filter set appropriate for both emission wavelenghts (CMFDA and CMTMR). (Data from 10 experiments. Ly, lymphocyte; Er, erythroblast; Mo, monocyte.). Single-dye polykaryocytes result from the fusion of cells stained by the same fluorescent dye, while dual-dye polykaryocytes result from the fusion of cells stained bt two different fluorescent dyes.

ISSN: 1475-2867