Figure 4

Changes in expression of apoptosis-related protein in response to treatment chloroform extract and quantification by scanning densitometry of PARP bands intensity. K562 cells were treated with 760, 380 and 190 μg/ml of chloroform extract for 6, 24 and 48 h. Protein extracts were subjected to western blotting to determinate immunoreactivity of PARP, as described in methods section. PARP 116 KDA and 85 KDA bands are shown. (quantified band intensity by computer-assisted densitometric analysis (Densitometer, GS- 800, BioRad Quantity One)). A: Untreated cells, B: 190 μg/ml, C: 38 0 μg/ml, D: 760 μg/ml, E: 190 μg/ml, F: 380 μg/ml, G: 780 μg/ml, H: 190 μg/ml, I: 380 μg/ml, J: 760 μg/ml.