Table 1 List of the primers used in RT-PCR and real-time PCR for Notch 1 gene amplification
From: Mutations increased overexpression of Notch 1 in T-cell acute lymphoblastic leukemia
Primer 5,6 | Sequence | Domain | PCR | Function |
|---|---|---|---|---|
notch26 + 27-F | 5'-ACGACCAGTACTGCAAGGACC - 3' | HD/exon 26 + 27 | RT-PCR | Sense |
notch26 + 27-R | 5'- AAGAACAGAAGCACAAAGGCG - 3' | Â | Â | Antisense |
notch28-F | 5'- TCGCTGGGCAGCCTCAACATCC - 3' | HD/exon28 | RT-PCR | Sense |
notch28-R | 5'- ACTCATTCTGGTTGTCGTCC - 3' | Â | Â | Antisense |
Notch TAD-F | 5'- GCCCTCCCCGTTCCAGCAGTCT - 3' | TAD | RT-PCR | Sense |
Notch TAD-R | 5'- GCCTGGCTCGGCTCTCCACTCA - 3' | Â | Â | Antisense |
Notch PEST-F | 5'- CAGATGCAGCAGCAGAACCTG - 3' | PEST/exon34 | RT-PCR | Sense |
Notch PEST-R | 5'- AAAGGAAGCCGGGGTCTCGT - 3' | Â | Â | Antisense |
Notch1-f | 5'-GCGACAACGCCTACCTCT-3' | Â | real-time PCR | Sense |
Notch1-r | 5'-CTGCTGGCACAGTCATCC-3' | Â | Â | Antisense |
β2M-f | 5'-TACACTGAATTCACCCCCAC-3' |  | real-time PCR | Sense |
β2M-r | 5'-CATCCAATCCAAATGCGGCA-3' |  |  | Antisense |