Figure 1

Effects of silencing of PLCG2 and CALM1 expression on cancer cell viability. 50,000 of HeLa cells from the exponentially growth phase were seeded in each of the wells of a 24-well plates the day before the siRNA/apatite complexes were prepared by mixing 3 μl of 1 M CaCl₂ with 10 nM of siRNA in 1 ml of fresh serum-free HCO₃^- (44 mM)-buffered DMEM medium (pH 7.5) and incubating at 37 °C for 30 min. The medium containing the siRNA/apatite complexes supplemented with 10% FBS had been added onto the rinsed cells before the cells were cultured consecutively for 48 h and the assessment on cell viability was carried out. Each experiment was done in triplicate and the data represent mean value ± SE (n = 3).