Adherent culture in serum-containing medium on plastic dishes coated with extracellular matrices. A: Coated with Matrigel™. B: Coated with collagen IV. C: Coated with laminin. D: Coated with fibronectin. E: Strong, positive, GFAP stain was noted (in red). Immature neurons positive for β-Tubulin III (green) were around the neurosphere (lower right) when grown on Matrigel™ in serum-containing medium. Nuclei were stained blue by Hoechst. (A, B, C, D: 10x objective; E, 20x objectives). The conclusion for panels A, B, C, D is that the cells behave differently in different adhesion molecules coated dishes. They grew in different speeds: from fast to slow – Matrigel™ (A) → Laminin (C) → Collagen IV (B) → Fibronectin (D).