Figure 2
From: HOXB1 restored expression promotes apoptosis and differentiation in the HL60 leukemic cell line
Effects of HOXB1 restored expression in HL60 cell line. Analysis of cell growth (a) and percentage of apoptotic & dead cells, as evaluated at day 7 of culture by the Annexin/PI analysis system in 10% (b) and 1% (c) FBS, *p < 0.01. Activation of CASPASE 3/7 in LXSN- and HOXB1-transduced HL60 cells in low serum condition and in presence of 200 nM staurosporine in 10% FBS (d), **p <0.005. Western blot analysis of pro- and active CASPASE 3 and APAF1 (e left) and BCL-2, BAX and MCL1 proteins (e right). Actin was used for normalization. Cell cycle analysis in LXSN- and HOXB1-transduced HL60 cells (f).