Presence of autocrine PRL in human cervical cancer cell lines. SiHa, C-33A, HeLa (Cervical cancer cells) and control cells MCF-7, T-47D (breast cancer), HaCaT (Inmortalized human keratinocytes) were cultured in DMEM or RPMI medium containing 10% FBS. A) PRL protein was determined by western blot using a specific antibody against PRL. B) Demonstration of the arbitrary optical density measurements from Western immunoblots assessing PRL levels. C) The cells grown on coverslips were fixed, and the localization of PRL (green) was observed by inmunocitochemistry using a secondary antibody conjugated with Alexa fluor 488 and DAPI stain (blue) to visualize the presence of cells. Magnification 40 x. D) Relative expression of PRL mRNA was measure by quantitative RT-PCR.