Figure 3

MiR-99a inhibits the apoptosise of HL60 and K562 cells. Apoptosis assessment by Annexin V/propidium iodide staining shows the apoptotic rates of K562 and HL60 cells transfected with miR-99a mimics, inhibitor-miR-99a or corresponding NC respectively. The fourth quadrant represents apoptotic cells detected by flow cytometry. (A, B) Of the K562 cells measured at 72 h after transfection, miR-99a inhibits apoptosis and the inhibition rate is 50.3%. (A) miR-99a NC, (B) miR-99a mimics. P < 0.05. (C, D) Of the K562 cells at 48 h, inhibitor-miR-99a prevents the inhibition of apoptosis and the prevention rate is 24%. (C) inhibitor-miR-99a NC, (D) inhibitor-miR-99a mimics. P > 0.05. (E, F) Of the HL60 cells at 96 h, miR-99a inhibits apoptosis and the inhibition rate is 18.5%. (E) miR-99a NC, (F) miR-99a mimics. P > 0.05. (G, H) Of the HL60 cells at 48 h, inhibitor-miR-99a prevents the inhibition of apoptosis and the prevention rate is 50%. (G) inhibitor-miR-99a NC, (H) inhibitor-miR-99a mimics. P < 0.05.