Figure 5

WT1 regulates AKT expression through promoter binding. A. The putative promoter region of AKT was predicted using the promoter scan website (http://www-bimas.cit.nih.gov/molbio/proscan). The WT1 binding motif in AKT promoter was predicted using the promoter and Transcription Factor Analysis web site (http://www.cbil.upenn.edu/cgi-bin/tess/tess), and was highlighted in red. B. Binding of WT1 to WT1 binding site in AKT gene promoter in vivo by ChIP assay. Genomic DNA and input chromatin (input), which represent portions of sonicated chromatin before immunoprecipitation, were both used as positive controls.