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Figure 1 | Cancer Cell International

Figure 1

From: Role of the Hypoxia-inducible factor-1 alpha induced autophagy in the conversion of non-stem pancreatic cancer cells into CD133+ pancreatic cancer stem-like cells

Figure 1

Characterization of morphology and stemness-related markers between pancreatic cancer stem-like cells and non-stem pancreatic cancer cells. (A) Phase-contrast images of the pancreatic cancer stem-like cells displayed epithelial-like appearance, while non-stem pancreatic cancer cells displayed fibroblast-like appearance. (B) Oct-4, c-MYC genes expression in pancreatic cancer stem-like cells and non-stem pancreatic cancer cells were detected by RT-PCR. (C) Expression of Oct-4, c-MYC proteins in pancreatic cancer stem-like cells and non-stem pancreatic cancer cells were detected by Western blotting. Data were normalized to β-actin levels. Experiments were repeated three times with similar data(* P < 0.05, **P < 0.01, ***P < 0.001). C represents for pancreatic cancer stem-like cells, N represents for non-stem pancreatic cancer cells. Scale bar equal 50 μm. (D) Oct-4, c-MYC gene expression of the bulk pancreatic cells under the intermittent hypoxia (IH) and normoxia (N) conditions were detected by RT-PCR. (E) Expression of Oct-4, c-MYC proteins in the bulk pancreatic cells under the intermittent hypoxia (IH) and normoxia (N) conditions were detected by Western blotting. Data were normalized to β-actin levels. Experiments were repeated three times with similar data (* P < 0.05, **P < 0.01, ***P < 0.001).

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