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Figure 2 | Cancer Cell International

Figure 2

From: Role of the Hypoxia-inducible factor-1 alpha induced autophagy in the conversion of non-stem pancreatic cancer cells into CD133+ pancreatic cancer stem-like cells

Figure 2

Intermittent hypoxia reprogrammed non-stem pancreatic cancer cells towards a Stem-like Phenotype. (A) Phase-contrast images of non-stem pancreatic cancer cells cultured under intermittent hypoxia (IH) and normoxia (N) condition. In intermittent hypoxia condition spheres can be detected while the rate of control cells in normoxia was few. (B) Oct-4, c-MYC gene expression of non-stem pancreatic cancer cells under the intermittent hypoxia (IH) and normoxia (N) conditions were detected by RT-PCR. (C) Expression of Oct-4, c-MYC proteins in non-stem pancreatic cancer cells under the intermittent hypoxia (IH) and normoxia (N) conditions were detected by Western blotting. Data were normalized to β-actin levels. Experiments were repeated three times with similar data (**P < 0.01, ***P < 0.001).

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Cancer Cell International

ISSN: 1475-2867

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