Primitive cells from HL-60 human myeloid leukemic cells are affected by H
. BM, UCB and HL-60 cells stimulated 1, 5 and 10 μM of H2O2 in vitro were labeled with specific mAbs for identification of the primitive subsets. (A) Representative dot plots from the gate strategy analysis used to select the populations of interest: m-HPC (Lin-Flk2-CD90LowSca-1-c-Kit+), m-HSC (Lin-Flk2-CD90LowSca-1+c-Kit+), h-HPC and LPC (CD34+), h-HSC and LSC (CD34+Lin-). (B and C) H2O2 did not alter the percentages of both murine and human hematopoietic progenitors and HSC (D) H2O2 reduces the LPC population and increases the LSC population. The data are expressed as the mean ± SEM of independent experiments performed in duplicate. BM n = 5, UCB and HL-60 n = 3 (3 independent experiments in duplicate). *P < 0.05, ANOVA.