Figure 2

Primitive cells from HL-60 human myeloid leukemic cells are affected by H ₂ O ₂ . BM, UCB and HL-60 cells stimulated 1, 5 and 10 μM of H₂O₂ in vitro were labeled with specific mAbs for identification of the primitive subsets. (A) Representative dot plots from the gate strategy analysis used to select the populations of interest: m-HPC (Lin^-Flk2^-CD90^LowSca-1^-c-Kit⁺), m-HSC (Lin^-Flk2^-CD90^LowSca-1⁺c-Kit⁺), h-HPC and LPC (CD34+), h-HSC and LSC (CD34⁺Lin^-). (B and C) H₂O₂ did not alter the percentages of both murine and human hematopoietic progenitors and HSC (D) H₂O₂ reduces the LPC population and increases the LSC population. The data are expressed as the mean ± SEM of independent experiments performed in duplicate. BM n = 5, UCB and HL-60 n = 3 (3 independent experiments in duplicate). *P < 0.05, ANOVA.