Figure 4
AKT phosphorylation is decreased by H 2 O 2 stimulus in total HL-60 cells. The cells were stimulated with 5 μM of H2O2, fixed, permeabilized and labeled with anti-phospho proteins to verify the activated status of ERK1/2, AKT and PLCγ2 by flow cytometry (A-C) BM, UCB and HL-60 cells and in the (D-F) stem cell subsets of each population. The fluorescence intensity of p-ERK1/2, p-AKT and PLCγ2 measured showed a significant decrease of p-AKT in total HL-60 cells. The data are presented as Gm. The data are expressed as the mean ± SEM of 3 independent experiments performed in duplicate. *P < 0.05, ANOVA.