Figure 2From: Restoration of klotho gene expression induces apoptosis and autophagy in gastric cancer cells: tumor suppressive role of klotho in gastric cancerThe role of demethylating agent and autophagy inhibitor on klotho protein expression and apoptosis. A) Representative Western blot of klotho protein expression. Demethylating agent 5-Aza upregulates klotho levels in a dose-dependent manner. B) Relative klotho protein levels in A). *p < 0.01, **p < 0.001 vs. 0 μmol/L of 5-Aza group. N = 5. C) Flow cytometry of apoptotic cells in GC-7901 cell under treatment of demethylating agent 5-Aza and autophagy inhibitor 3-MA. D) The percentage of apoptotic cells under treatment of 5-Aza and 3-MA. *p < 0.01 vs. control; #p < 0.05 vs. 5-Aza treatment. N = 5.Back to article page