The role of demethylating agent and autophagy inhibitor on klotho protein expression and apoptosis. A) Representative Western blot of klotho protein expression. Demethylating agent 5-Aza upregulates klotho levels in a dose-dependent manner. B) Relative klotho protein levels in A). *p < 0.01, **p < 0.001 vs. 0 μmol/L of 5-Aza group. N = 5. C) Flow cytometry of apoptotic cells in GC-7901 cell under treatment of demethylating agent 5-Aza and autophagy inhibitor 3-MA. D) The percentage of apoptotic cells under treatment of 5-Aza and 3-MA. *p < 0.01 vs. control; #p < 0.05 vs. 5-Aza treatment. N = 5.