Figure 3

The role of demethylating agent and autophagy inhibitor on autophagy. A) Representative of Western blot of LC3-I and LC3-II protein expression. Demethylating agent 5-Aza upregulated LC3-II and downregulated LC3-I levels in a dose-dependent manner. B) Relative LC3-II/LC3-I ratio in A). ^*p < 0.01, ^**p < 0.001 vs. 0 μmol/L of 5-Aza group. N = 5. C) Representative of Western blot of LC3-I and LC3-II protein expression under treatment of 5-Aza with or without 3-MA treatment. D) Relative LC3-II/LC3-I ratio in C). ^*p < 0.01, ^**p < 0.001 vs. 0 μmol/L of 5-Aza group. N = 5.