Figure 3

Suppression of the expression of p53 and Bak by miR-125b. A) miR-125b was stably knocked down in Dox-resistant EWS cells, and the whole cell lysates were subjected to an immunoblot analysis using antibodies against P-gp and β-actin. B), C) miR-125b was stably knocked down in EWS cells, and the cells were incubated with or without 100 ng/ml of doxorubicin for 24 h. The whole cell lysates were subjected to an immunoblot analysis using antibodies against caspase 3, cleaved caspase 3, and β-actin (B). The active caspase 3 levels were quantitated by using ELISA (C). D) miR-125b was stably knocked down in EWS cells, and the whole cell lysates were subjected to an immunoblot analysis using antibodies against p53, Bak, and β-actin.