Effect of down-regulation of Beclin1 on proliferate viability in Miapaca2 cells. A. Miapaca2 cells transfected with pGPU6/GFP/Neo-shNC for 24 h by Lipofectamine 2000 exhibited green fluorescent signals under an inverted fluorescence microscope. B. Miapaca2 cells were stably transfected with pGPU6/Neo-shBECN1 or pGPU6/Neo-shNC for 48 h, then exposed to G418 (400ug/ml) for 18 days. Western blot analyze of the Beclin1 expression. C. Small RNA interference of Beclin1 had not obvious influence on survival of Miapaca2 cells in 96 h. Cell viability was determined by the Cell counting Kit-8 assay. The difference was not significant between Beclin1-siRNA group and NC-siRNA group (p > 0.05).