Figure 2

AZD6244 inhibited HCC cell growth and enhanced chemosensitivity. A: HepG2 and Huh7 were treated with four different concentrations (5 μM, 10 μM, 15 μM and 20 μM) of MEK inhibitor U0126 and AZD6244. After 48 hours treatment, cell viability test was performed to measure the relative viability. * P<0.05 compared with control group. B: HepG2 and Huh7 cells were treated with U0126 (10 μM) and AZD6244 (10 μM) for 24 hours, followed by gemcitabine (50 μg/ml, 100 μg/ml, 150 μg/ml and 200 μg/ml) or doxorubicin (0.15 μg/ml, 0.30 μg/ml, 0.45 μg/ml and 0.6 μg/ml) for another 48 hours. Cell viability test was performed to measure the relative viability. *: synergistic effect was detected.