Figure 1

PI3K/Akt signalling is constitutively activated in A204 and A673 cell lines. Protein levels and phosphorylation status of Akt and β-actin (loading control), were analyzed by Western blotting in A. 24 hours cell culture of A204 cells under normoxia, treated with 0, 10, 20, 30 μmol/L LY294002. B. 24 hours cell culture of A673 cells under normoxia, treated with 0, 10, 20, 30 μmol/L LY294002. C. 24 h culture of A204 cells under normoxia or hypoxia, medium containing either 0% (−) or 10% FCS (+; top) and/or treated with 30 μmol/L LY294002. D. 24 h culture of A673 cells under normoxia or hypoxia, medium containing either 0% (−) or 10% FCS (+; top) and/or treated with 30 μmol/L LY294002. E. Western blots obtained as indicated in C were densitometrically analyzed. Shown are means ± SEM of four independent experiments. Statistically significant differences between LY294002 treated and untreated (20% FCS) are indicated *, p < 0.05 and **, p < 0.01. Statistically significant differences between LY294002 treated and untreated (0% FCS) are indicated #, p < 0.05 and ##, p < 0.01. The difference between untreated hypoxic and normoxic condition was not significant (for 20% FCS, p = 0.1288; for 0% FCS, p = 0.8749). F. Western blots obtained as indicated in D were densitometrically analyzed. Shown are means ± SEM of four independent experiments. Statistically significant differences between LY294002 treated and untreated (20% FCS) are indicated *, p < 0.05 and **, p < 0.01. Statistically significant differences between LY294002 treated and untreated (0% FCS) are indicated #, p < 0.05 and ##, p < 0.01. The difference between untreated hypoxic and normoxic condition was not significant (for 20% FCS, p = 0.1529; for 0% FCS, p = 0.3275).