Figure 2

Bisulfite specific PCR sequencing to assess CpG islands of miR130b. Box plots showed the mean ± SD. A. Relative methylation levels in Ishikawa cells treated with 5’-Aza-Cdr (10 μM) for 48 h, TSA (10 μM) for 48 h, or 5’-Aza-Cdr for 48 h plus TSA for 24 h. B. Relative methylation levels in AN3CA cells treated with 5’-Aza-Cdr (10 μM) for 48 h, TSA (10 μM) for 48 h, or 5’-Aza-Cdr for 48 h plus TSA for 24 h. (C) Different levels of methylation between normal endometrium and EECs.