Figure 5

GFAP immunoreactivity. (A) Graph showing the average number of GFAP positive cells in 4 areas of the cortex (0.0678 mm²) in animals injected with culture medium, or injected with CRCTU or ATCC Walker 256 cells into the internal carotid artery; **p<0.01; *p<0.05. (B) Graph showing the average number of GFAP positive cells in 4 areas of the striatum (0.0678 mm²) following direct inoculation of culture medium, CRCTU or ATCC Walker 256 cells into the brain; **p<0.01. (C) GFAP immunostained section 9 days following CRCTU Walker 256 internal carotid artery injection, showing absence of staining within the tumour, but showing GFAP labelled cell in peritumoral area (arrows). (D) Brain section stained for GFAP 10 weeks following carotid ATCC Walker 256 tumour cell injection. Labelled astrocytes (arrows) are seen in the peritumoral area and in the periphery of the tumour mass between the tumour cells. (E) High magnification of GFAP immunostained section 9 days following CRCTU Walker 256 internal carotid artery injection. (F) High magnification of GFAP immunostained section 10 weeks following ATCC Walker 256 internal carotid artery injection. (G) GFAP immunostained section 7 days following direct inoculation of CRCTU Walker 256 inoculation showing substantial peritumoral immunoreactivity.