Figure 5
Expression of proteins changed in cells treated with different concentrations of apigenin or apigenin and LY294002 for 24 h. (A) Apigenin reduced the expression phosphorylation of Akt, PI3K and PDK significantly, but the expression of total Akt had no change. (B) T24 cells was treated with 20 μM PI3 kinase inhibitor LY294002 for 30 min and then with 40 μM apigenin for another 24 h. PI3K inhibitor, LY294002 decreased the protein levels of cleaved PARP and active casepase-3 which were activated by apigenin. (C) Apigenin inhibited the expression of anti-apoptotic gene products Bcl-2 and Bcl-xL but upregulated the expression of apoptotic gene product Bax and Bad. A representative blot is shown from three independent experiments with identical results.