Figure 10

Co-localization of BRCA1 antibody (K- 18) and nucleolin (7G2) in MCF7 cells. A. Rabbit polyclonal BRCA1 antibody stains many cell nuclei (green). The varied nuclear patterns include many small dot-like speckles, punctate bodies and speckles, and round centrally placed bodies. B. Mouse monoclonal antibody to nucleolin stains nucleoli and many dot-like speckles (red). C. Nuclei which stain for BRCA1 (green) merge with nucleolin (red) to show co-localization(yellow). The merge occurs both in nucleoli (which are phase dense in D) and in some nuclear speckles. Not all of the nucleoli show co-localization. D. Nomarski image of the same field as A,B,C. Bar = 20 μm.