Table 1 Antibodies
Antigen | Cat. # | Source | Dilution | Application |
|---|---|---|---|---|
E-cadherin | 610181 | BD Biosciences | 1:2000 | WB |
1:50 | IF | |||
N-cadherin | 610910 | BD Biosciences | 1:2000 | WB |
β-actin | 4967 | Cell Signaling | 1:4000 | WB |
ZO-1 | 339100 | Invitrogen | 1:100 | IF |
K5 | PRB-160 | Covance | 1:100 | IF |
K8 | GP11 | Progen Biotechnik | 1:50 | IF |
ER (MC20) | SC545 | Santa Cruz | 1:200 | IHC |
AlexaFluor 568 conjugated phalloidin | A12380 | Invitrogen | 1:40 | IF against actin |