Cell cycle analysis after 24 hour exposure to ESE-16. (2i) Histograms derived from flow cytometry comparing cell distribution within the cell cycle between control samples and ESE-16 treated cells. (2ia) Cell cycle distribution of cells grown in medium only. (2ib) Cells exposed to DMSO as a vehicle control. (2ic) Cells exposed to actinomycin D as a positive apoptosis control. (2id) Cells exposed to ESE-16 demonstrating an increase in G2/M and sub-G1 populations with a concurrent decrease in G1- and S phase. (2ii) Graphical representation of the histogram results. (* indicates a statistically significant difference when compared to DMSO vehicle control samples with a P value of < 0.05). Standard deviation is indicated by T-bars.