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Figure 4 | Cancer Cell International

Figure 4

From: Wnt5a promotes migration of human osteosarcoma cells by triggering a phosphatidylinositol-3 kinase/Akt signals

Figure 4

Effect of Akt in Wnt5a-induced cell migration. (A) Effect of Akt siRNA on the expression of Akt. MG-63 cells were transiently transfected with Akt siRNA pool, or mock for 48 h. Cells were analyzed by immunoblotted with total Akt antibody. (B) Effect of Akt siRNA on Wnt5a-stimulated cell migration. Cells were transiently transfected with Akt siRNA pool, and stimulated with 100 ng/ml Wnt5a or not for 10 h. Relative cell migration rate was determined by using wound healing assay. ** p < 0.01 in the cultures with Wnt5a relative to the cultures without Wnt5a. Data were presented as mean ± SD of 5 determinations.

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Cancer Cell International

ISSN: 1475-2867

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