Figure 1

β4 integrin expression in NMuMG cell variants. Subconfluent NMuMG monolayers were treated with TGFβ1 (5 ng/ml) for 48 hours. Total RNA was extracted and reverse-transcribed to cDNA. β4 integrin was determined using quantitative real-time polymerase chain reaction (qRT-PCR) and normalized to GAPDH levels. Graph depicts the average fold change (± SEM) in Par6-expressing (Par6/wt and Par6/S345A) and an empty vector-expressing clonal variant of NMuMG cells (NMuMG-V1; later referred to as β4 null) relative to parental cells; n = 3 for biological replicates and n = 3 for technical replicates. Two-way ANOVA for all cell lines and treatments was significant (p < 0.0001 and p = 0.0060, respectively). Bonferroni post test compared differences between control and treatment for each cell line, ***p <0.0001.