Figure 4

miR-125a-5p could mediate the anti-proliferation effect of gefitinib on NPC cells. (A). Using oligo-miR-nc (FAM) as an example, a FAM reporter assay confirmed that the miRNA oligos used in this study were successfully transfected into HNE-1 and HK-1 cells. qRT-PCR revealed that the relative quantities of miR-125a-5p were decreased in cells transfected with oligo-miR-125a-5p inhibitor (P < 0.05), and miR-125a-5p was increased in cells transfected with oligo-miR-125a-5p mimic (P < 0.05). (B). CCK-8 assay showed that the anti-proliferative effect of gefitinib on HNE-1 and HK-1 cells was weakened by transfection with oligo-miR-125a-5p inhibitor and promoted by transfection with oligo-miR-125a-5p mimic. The results are representative of three individual experiments.* P < 0.05.