Effect of Nkx2.8 overexpression on HCC cell function. (A, B) The detection of Nkx2.8 overexpression in PLC and Huh7 cells by WB and q-RT-PCR 48 h after transfection, respectively. (C, D) The cell proliferative activities were determined in PLC and Huh7 cells by a MTT assay. Each column represents the mean ± S.D. for three independent experiments. (E) A representative result of the colony formation assays for the effects of Nkx2.8 on the in vitro proliferation abilities of PLC and Huh7 cells. The results represent the mean ± S.D. for four independent experiments. *p < 0.05.