Figure 1
From: Anti-cancer activity of glucosamine through inhibition of N-linked glycosylation
Glucosamine reduced the molecular mass of gp130 and suppressed the phosphorylation of STAT3 in DU145 cells. (A) Western blot analysis of the extracts from the cells cultured in the presence of 2 mM glucosamine for the indicated time (h). Whole-cell lysates were subjected to immunoblotting using antibodies specific for gp130 and actin (loading control). The open arrow indicates the molecular mass of gp130 without glucosamine treatment and the filled arrow indicates the reduced molecular mass of gp130 following the treatment. (B) Western blot analysis of cells cultured with indicated concentrations of glucosamine (mM) for 24 h. Whole-cell lysates were subjected to immunoblotting using antibodies specific for gp130, phospho (Tyr705)-STAT3 (p-STAT3), STAT3 and actin (loading control). (C) Western blot analysis of cells cultured with indicated concentrations of tunicamycin (μM) for 24 h. Whole-cell lysates were subjected to immunoblotting using the same antibodies as described for B. Each blot is a representative of three independent experiments.