Figure 1
From: Caveolin-1 induces lamellipodia formation via an Akt-dependent pathway
Caveolin-1 enhances lamellipodia formation in lung cancer H23 cells. (A) Cells were transfected with Cav-1 overexpressing, control, shCav-1 or shRNA scrambled plasmids and analyzed for Cav-1 level by western blotting. The blots were reprobed with β-actin to confirm equal loading. The immunoblot signals were quantified by densitometry, and mean data from independent experiments were normalized to the results. The bars are the means ± SD (n = 4). *, p < 0.05 vs. control (H23/Ctrl) or shRNA scrambled (H23/shCtrl) transfected cells. (B) Lamellipodia in the transfected cells was indicated by arrow. Data represent as relative number of lamellipodia/cell as described in Material and Methods. Plots are mean ± SD (n = 4). *, p < 0.05 vs. control (H23/Ctrl) or shRNA scrambled (H23/shCtrl) transfected cells. Lamellipodia at the edge of cells were indicated by arrow. (C) Transfected cells were stained with phalloidin-rhodamine and visualized under fluorescent microscope.