Figure 1

Flow cytometric output for the EGFR and ErbB4 receptors screening in the H103 cell line. The true percentage of cells expressing EGFR or ErbB4 receptors were calculated by subtracting the autofluorescence of the unstained cells (B and D) from the fluorescence of the cells stained with the anti-EGFR-FITC or anti-ErbB4-PE antibodies (C and E) respectively. A The polygonal box, R₁ represents the gating of the cells based on the forward and side scatter profiles to exclude cellular debris and doublet cells. A total of 10,000 gated events were acquired for analysis. B The green dots within the R₂ box represent the autofluorescence of unstained cells. This autofluorescence was subtracted from the fluorescence detected in C. C The green dots within the R₂ box represent the fluorescence of cells after staining with anti-EGFR-FITC antibodies. D The pink dots within the R₃ box represent the autofluorescence of unstained cells. This autofluorescence was subtracted from the fluorescence detected in E. E The pink dots within the R₃ box represent the fluorescence of cells after staining with anti-ErbB4-PE antibodies.