Table 1 Primers used for real-time PCR amplifications
From: Curcumin inhibits leptin gene expression and secretion in breast cancer cells by estrogen receptors
Primer | Primer length | Sequence (5′ to 3′) | Product size (bp) |
|---|---|---|---|
Leptin forward | 22 | CACCAAAACCCTCATCAAGACA | 80 |
Leptin reverse | 24 | CTTTCTGTTTGGAGGAGACTGACT | |
ERα forward | 20 | GCCAGCAGGTGCCCTACTAC | 132 |
ERα reverse | 23 | TGGTACTGGCCAATCTTTCTCTG | |
ERβ forward | 19 | AAGAGCTGCCAGGCCTGCC | 268 |
ERβ reverse | 21 | GCGCACTGGGGCGGCTGATCA | |
Î’-actin forward | 20 | TGGACTTCGAGCAAGAGATG | 137 |
Î’-actin reverse | 20 | GAAGGAAGGCTGGAAGAGTG |