Figure 2

Analysis of internucleosomal DNA fragmentation. A: Agarose gel electrophoresis of low molecular weight DNA extracted from HeLa and MON cells exposed for 2 h to 10 μM CisPt (P) or 10 μM etoposide (E) or irradiated with 5 Gy of X-rays (X) and harvested on consecutive days thereafter. C refers to untreated controls. B: Agarose gel electrophoresis of low molecular weight DNA extracted from HeLa and MON cells exposed to 5, 10 or 20 J/m² UVC or not irradiated (0) and harvested on consecutive days thereafter. In A and B, low molecular weight DNA was extracted from 2.10⁶ cells at each time and analyzed by electrophoresis through a 2% agarose gel. Sizes of DNA molecular weight standards are indicated. Gels shown are representative of three separate experiments.