Figure 5
Western blot analysis of relevant proteins involved in stress response and apoptosis in MON cells irradiated with 4 Gy of X-rays (X) or exposed to 2 (W2) or 4 μM wortmannin (W4), 8 nM vinblastine (V), 4 μM CisPt (Pt) or by the combined treatments 4 Gy X-rays plus 2 (X+W2) or 4 μM wortmannin (X+W4), 4 Gy X-rays plus 4 μM CisPt (X+Pt), 4 Gy X-rays plus 8 nM vinblastine (X+V), 8 nM vinblastine plus 2 (V+W2) or 4 μM wortmannin (V+W4), 4 μM CisPt plus 2 (Pt+W2) or 4 μM wortmannin (Pt+W4), and 4 μM CisPt plus 8 nM vinblastine (Pt+V). C refers to the untreated control. Lysates from 5.105 cells were subjected to 7–14% gradient SDS-PAGE, blotted and incubated with the appropriate antibodies. The data are representative of two independent experiments.