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Figure 7 | Cancer Cell International

Figure 7

From: Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line

Figure 7

PP1 activity in lysates of cells expressing 6His-HA-PP1α. Cells were cultured for 24 hr either in the absence (closed diamonds) or presence (closed squares) of doxycycline. PP1 was assayed using the indicated amounts of lysate/assay. 5 nM okadaic acid was used to inhibit PP2A during the assay. 1 unit of PP1 activity releases 1 nmol of Pi/min at 30°C.

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