Figure 6

Effect of EGF and/or Li on the subcellular distribution of β-catenin. The level of β-catenin expressed under EGF and/or Lithium stimulation are expressed as a fold increase relative to a) the membrane standard (= 1, indicated by the red line) for the membrane fractions, and b) to the SNO standard (= 1, indicated by the red line) for the free fractions. a) In general, EGF and Li alone slightly increase the levels of β-catenin. Combined EGF and Li signalling results in a decline of membrane-associated β-catenin. b) Little or no β-catenin was detected in the free fractions after exposure to EGF (3 hours), Li (12 and 24 hours), or EGF and Li (12 and 24 hours). Li stimulation (3 hours) increased free levels in WHCO1, WHCO3 and WHCO5. Concurrent EGF and Li treatment (3 hours) caused free β-catenin to increase substantially in all cell lines, most notably in WHCO3 (over 8-fold).