Figure 6From: Phorbol myristate acetate and Bryostatin 1 rescue IFN-gamma inducibility of MHC class II molecules in LS1034 colorectal carcinoma cell lineEffect of protein kinase inhibitors on physiological and PMA potentiated responses to IFNg. Expression levels of MHCII induced by 1,000 IU/ml IFNg alone are indicated by triangles (▲). Expression levels of MHCII induced by combined treatment with 1,000 IU/ml IFNg, 1,000 ng/ml PMA and 172 mM ethanol are indicated by squares (■). IFNg and PMA were added after cells had been pretreated with Staurosporine and GF 109203X for 1 hr. Incubation continued for the next 24 hr in the constant presence of inhibitors. Physiological response to IFNg in SW480 colon carcinoma cells was abrogated by 1 μM Staurosporine. PMA-potentiated response to IFNg in LS1034 colon carcinoma cells was abrogated by 100 nM Staurosporine or by 1 μM GF 109203X. Mean values of two independent experiments are plotted.Back to article page