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Figure 3 | Cancer Cell International

Figure 3

From: Chemosensitivity assay in mice prostate tumor: Preliminary report of flow cytometry, DNA fragmentation, ion ratiometric methods of anti-neoplastic drug monitoring

Figure 3

Dependence of DNA fragmentation on drugs and cell lines. Using the APO-BRDU endlabelling technique evaluated various antineoplastic agents (VP16 etoposide, 9-amino-camptothecin, cisplatin) at various times (8 and 24 hrs). Graph displays the fluorescent intensity (per 10000 cells) during different experimental conditions (3A) and illustrate that, at standard tissue culture doses: i) there was a minimal response at 8 hrs; ii) a range of responses for a given cell lines (DU145 response was highest with VP16, and not detectable with cisplatin); and, iii) for same drug and dose (VP16 at 10 μg/ml) DU145 response was greater than for PC3. Sample histograms showed fluorescence intensity distribution for untreated control cells (3B) and after a 24 hours VP16 treatment (3C).

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Cancer Cell International

ISSN: 1475-2867

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