Skip to main content
Figure 4 | Cancer Cell International

Figure 4

From: Human breast cancer cell-mediated bone collagen degradation requires plasminogen activation and matrix metalloproteinase activity

Figure 4

RT-PCR of uPA and uPAR in breast cancer cells. ZR-75-1 breast cancer cells were cultured as described in the material and methods in the absence (A) and presence (B) of TGFβ (10-10 M). Total RNA was isolated and RT-PCR performed with specific primers for uPA, tPA and uPAR. The housekeeping gene GAPDH was used as a positive control. Lane 1 uPA; lane 2 uPAR; lane 3 GAPDH. The normal breast cell line HME stimulated with TGFβ is shown in (C). Band intensities were quantified by scanning densitometry and data expressed as a ratio (uPA or uPAR/G3PDH) of the average optical density (OD) × area. The ratio of the intensity of the uPA or uPAR mRNA band over the intensity of the G3PDH mRNA was arbitrarily designated as 1.0.

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us