1A. Rho GTPase protein expression levels in the IBC cell line SUM 149 versus SUM 102. 1B. F-actin and focal adhesion distribution in SUM 149 and SUM 102 human breast cancer cell lines. 1A. Equal protein amounts were separated by 10% SDS-PAGE, transferred to nitrocellulose, and probed for RhoA, RhoC, and Rho (A, B, and C). Images are representative of at least three independent experiments with actin serving to verify equal protein loading. 1B. SUM 149 and SUM 102 cells were starved in unsupplemented F-12 Hams media for 24 hours and stimulated for 10 minutes with PBS (control), EGF (50 ng/ml), or FBS (5%). Cells were stained with rhodamine phalloidin (red) to visualize F-actin and anti-phosphotyrosine (green) to visualize focal adhesion. Micrographs were taken at 1000× magnification. Images are representative for at least three independent experiments.