Figure 1

Immunoblot of U94 protein. Nuclear and cytoplasmic protein was extracted from confluent PC3 cell line, PC3 cell line transfected with vector cassette (controls) or PC3 cell line transfected with plasmid containing U94 DNA (test). 100 μg sample protein was loaded per lane and separated by SDS-PAGE through a 10% Tris-glycine gel (Novex; Invitrogen, Gaithersburg, MD). The results showed the 56 kDa U94 protein in the nuclear extract of U94 recombinant cells. There was no immunoreactivity in all cytoplasmic extracts, and nuclear extracts of control cells. U94 protein was detected using the polyclonal anti-U94 antibody, AB679 (Amersham, England). The positions of 46 kDa and 66 kDa markers, and the U94 protein are indicated on the right.