Figure 4
From: P53 and Beta-Catenin Activity during Estrogen treatment of Osteoblasts
Beta-catenin expression during E2 treatment: Immunostaining of E2 treated ROS-PG13 with anti-beta-catenin antibody demonstrates its localization within the cells at 24 and 48 h after treatment. Cells were grown on cover slips and treated with 10-11 M 17-beta estradiol in 2% charcoal treated serum containing media for the different lengths of time indicated. Control cells were grown in 2% charcoal treated serum containing media. Other details are as described under methods.