Determination of ILK isoform/s in HOSCCs. Amplified cDNA fragments were digested with either Bam H1 (B) or Hinc II (H) restriction enzymes. Non-digested (N) and digested fragments (B, H) were separated on 2% agarose gel electrophoresis and stained with ethidium bromide. (A) Bam H1 restriction analysis produced two fragments of 823 and 536 bp respectively whereas (B) Hinc II restriction analysis did not digest the 1360 bp ILK fragment. 1 kb ladder in lane 1.