Immunfluorescence localisation of ILK. Under standard tissue culture conditions ILK localised to both cytoplasmic and nuclear compartments (red arrows). ILK was detectable at focal adhesions (yellow arrows) in the (A) WHCO1, (B) WHCO3, (C) WHCO5, (D) WHCO6 and (E) SNO cell lines. ILK was stained utilising an anti-ILK antibody (1:500). Bar, 10 μm.