Figure 6
ILK is catalytically active in HOSCCs. ILK was immunoprecipitated using protein A sepharose beads (25 mg). MBP kinase assays were performed utilising 10 μCi [γ-32p] ATP and kinase reaction products were resolved on 10% SDS-PAGE subjected to autoradiography. (A) MBP phosphorylation in the WHCO1, WHCO3, WHCO5 WHCO6 and SNO cell lines. (B) Semi-quantitative ILK kinase activity established by densitometry of autoradiograms. The levels of MBP phosphorylated kinase products of WHCO1, WHCO3, WHCO5, WHCO6 and SNO. (C) The effect of 24 hours EGF and TGFβ1 exposure on ILK kinase activity in the WHCO1, WHCO3, WHCO5 and SNO cell lines. (D) Semi-quantitative ILK kinase activity established by densitometry of autoradiogams. Results are representative of three independent experiments.