Figure 3From: Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblastsExpression of E5 protein in vitro and cell line mRNA. (A) Autoradiographs of PAGE gels containing in vitro wheat germ protein translation products of HPV-16 E5 variants (G) and 'stop' codon controls (S), demonstrating 35S-labelled 9.1 kDa products in the former, but not the latter. +: positive (luciferase), and -: negative kit controls. X: 1 ng HPV-16 RNA ; Y: 0.1 ng HPV-16 RNA. (B) Expression of E5 mRNA detected by RT-PCR in transfected cell-lines analysed in the presence (+) or absence of reverse transcriptase. B: blank.Back to article page