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Figure 3 | Cancer Cell International

Figure 3

From: "Nutritional and chemopreventive anti-cancer agents up-regulate expression of p27Kip1, a cyclin-dependent kinase inhibitor, in mouse JB6 epidermal and human MCF7, MDA-MB-321 and AU565 breast cancer cells"

Figure 3

Deletion analysis indicates that various nutritional and chemopreventive anti-cancer agents activate the proximal 5'-upstream region (-1797) of p27 gene through its 5'-untranslated region (5'UTR). (a) Deletion mutants of -1797 p27 (p27-Kip I) used in this experiment: -1797 p27 (p27-Kpn I), -774 p27 (p27-Apa I), -575 p27 (p27-5'UTR), -435 p27 (p27-MB), and -417 p27 (p27-IRES). The (P+) JB6 mouse epidermal cells and estrogen receptor (ER)-negative MDA-MB-231 cells were transfected with these deletion mutants and then exposed to (b) 9-cis-retinoic acid (9cRA) (1 μM), (c) dexamethasone (1 μM), (d) 4-hydroxytamoxifen (258 nM), or (e) genistein (370 nM) for 24 hours. All assays were performed in triplicates and the transfection experiments were repeated three times.

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Cancer Cell International

ISSN: 1475-2867

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