Figure 7
Inhibition of phosphoinositide 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) also up-regulates the activity of -575 p27 (5'-untranslated region (5'UTR) of p27 gene). Furthermore, inhibition of the global methylation of 5'-m7G-cap of other mRNAs also up-regulates the activity of -575 p27 (5'-untranslated region (5'UTR) of p27 gene). The estrogen receptor (ER)-negative MDA-MB-231 cells, transfected with -575 p27 (p27-5'UTR), were treated with LY 294002 (3.25 μM) to inhibit PI3K; triciribine (3.12 μM) to inhibit Akt/PKB, rapamycin (1.09 μM) to inhibit mTOR; COX-1 inhibitor FR122047 (1.09 μM) and COX-2 inhibitor II (1.43 μM) to inhibit cyclooxygenases; NSC 119889 (7.70 μM) to competitively inhibit S-adenosylmethionine; or salubrinal (1.04 and 10.4 μM) to inhibit phosphatase of eukaryotic translation initiation factor 2α (eIF2α) for 24 hours. All assays were performed in triplicates and the transfection experiments were repeated three times.